Peter Parker / Spider-Man (voice)

Mary Jane Watson (voice)

Harry Osborn (voice)

J. Jonah Jameson (voice)

Officer Barr (voice)

Doug's friend (voice)

Muang / Drip Den guy (voice)

Max Dillon / Electro (voice)

Max Dillon (voice)

Sally Johnson (voice)

Silver Sable (voice)

Kraven the Hunter (voice)

Sergei (voice)

Raymond (voice)

Policeman (voice)

Professor (voice)

Dr. Zellner (voice)

Head Protestor (voice)

Shadowy Woman / Flower Vendor (voice)

Roland Gaines (voice)

Agent Mosely (voice)

Roxanne Gaines (voice)

Female News Announcer (voice)

Female Reporter (voice)

Indira 'Indy' Daimonji (voice)

Wilson Fisk / The Kingpin (voice)

Frank Elson (voice)

Dr. Curt Connors / The Lizard (voice)

Shikata (voice)

Richard Damien (voice)

Oscorp Executive (voice)

Christina (voice)

Flash Thompson (voice)

Cheyenne Tate / The Talon (voice)